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    A Phytophthora sojae G Protein α Subunit is Involved 1 In Ch

      

    Eukaryotic Cell doi:10.1128/EC.00286-08

    A Phytophthora sojae G-Protein  Subunit Is Involved in Chemotaxis to Soybean Isoflavones†
    Chenlei Hua,1 Yonglin Wang,1 Xiaobo Zheng,1 Daolong Dou,1 Zhengguang Zhang,1
    Francine Govers,2 and Yuanchao Wang1*
    Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China,1 and Laboratory of Phytopathology,
    Plant Sciences Group, Wageningen University, Binnenhaven 5, NL-6709 PD Wageningen, The Netherlands2
    Received 29 August 2008/Accepted 8 October 2008
    For the soybean pathogen Phytophthora sojae, chemotaxis of zoospores to isoflavones is believed to be critical
    for recognition of the host and for initiating infection. However, the molecular mechanisms underlying this
    chemotaxis are largely unknown. To investigate the role of G-protein and calcium signaling in chemotaxis, we
    analyzed the expression of several genes known to be involved in these pathways and selected one that was
    specifically expressed in sporangia and zoospores but not in mycelium. This gene, named PsGPA1, is a
    single-copy gene in P. sojae and encodes a G-protein  subunit that shares 96% identity in amino acid sequence
    with that of Phytophthora infestans. To elucidate the function, expression of PsGPA1 was silenced by introducing
    antisense constructs into P sojae. PsGPA1 silencing did not disturb hyphal growth or sporulation but severely
    affected zoospore behavior, including chemotaxis to the soybean isoflavone daidzein. Zoospore encystment and
    cyst germination were also altered, resulting in the inability of the PsGPA1-silenced mutants to infect soybean.
    In addition, the expressions of a calmodulin gene, PsCAM1, and two calcium- and calmodulin-dependent
    protein kinase genes, PsCMK3 and PsCMK4, were increased in the mutant zoospores, suggesting that PsGPA1
    negatively regulates the calcium signaling pathways that are likely involved in zoospore chemotaxis.


    发布时间: 2009-03-21 03:32 浏览次数:   【 打 印 】【 关 闭
     
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